Tuber melanosporum (T. melanosporum), which is known as the black truffle, is the most famous edible ectomycorrhizal (ECM) fungus with a great commercial interest in the world, due to the expensive delicacies and can develop symbiotically with the roots of various trees and some bushes. T. melanosporum was ascomycete hypogeous fungi, which forms an underground fruitbody. In this study, we infected the seedlings of Quercus acutissimar (cork oak), which is a main forest species grown naturally in DPR Korea, with T. melanosporum mycelium to identify the emerged mycorrhizal association morphologically and molecularly. It was shown that Q. acutissimar, selected as the host species, was compatible with T. melanosporum. One hundred of the Q. acutissimar seeds were allowed to germinate and germination was observed in 84 seeds. Among 84 germinated seedlings, 70 seedlings with relatively good growth were selected and inoculated with T. melanosporum mycelium. Characteristic mycorrhizal structures between T. melanosporum and Quercus acutissimar were observed in 52 seedlings. The identification of T. melanosporum mycorrhiza was based on morphological and anatomical features and confirmed with molecular analyses by PCR. This is the first report on the establishment of the ECM association between T. melanosporum mycelia with Quercus acutissimar seedlings in DPR Korea.
| Published in | Frontiers in Environmental Microbiology (Volume 11, Issue 3) |
| DOI | 10.11648/j.fem.20251103.12 |
| Page(s) | 62-65 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2025. Published by Science Publishing Group |
Tuber Melanosporum, Mycelial Inoculation, Quercus acutissimar, Mycorrhizal Fungi
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APA Style
Jang, K., Kim, Y., Kim, C., Hyon, B., Kim, H., et al. (2025). Developments of Mycorrhiza in Quercus acutissimar (Cork Oak) Seedlings Inoculated with Black Truffle (Tuber melanosporum) Mycelial Pure Cultures. Frontiers in Environmental Microbiology, 11(3), 62-65. https://doi.org/10.11648/j.fem.20251103.12
ACS Style
Jang, K.; Kim, Y.; Kim, C.; Hyon, B.; Kim, H., et al. Developments of Mycorrhiza in Quercus acutissimar (Cork Oak) Seedlings Inoculated with Black Truffle (Tuber melanosporum) Mycelial Pure Cultures. Front. Environ. Microbiol. 2025, 11(3), 62-65. doi: 10.11648/j.fem.20251103.12
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Download@article{10.11648/j.fem.20251103.12,
author = {Kum-dong Jang and Yong-nam Kim and Chol Kim and Bok-sil Hyon and Hyok Kim and Jong-chong Kim and Hui-won Kim},
title = {Developments of Mycorrhiza in Quercus acutissimar (Cork Oak) Seedlings Inoculated with Black Truffle (Tuber melanosporum) Mycelial Pure Cultures
},
journal = {Frontiers in Environmental Microbiology},
volume = {11},
number = {3},
pages = {62-65},
doi = {10.11648/j.fem.20251103.12},
url = {https://doi.org/10.11648/j.fem.20251103.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20251103.12},
abstract = {Tuber melanosporum (T. melanosporum), which is known as the black truffle, is the most famous edible ectomycorrhizal (ECM) fungus with a great commercial interest in the world, due to the expensive delicacies and can develop symbiotically with the roots of various trees and some bushes. T. melanosporum was ascomycete hypogeous fungi, which forms an underground fruitbody. In this study, we infected the seedlings of Quercus acutissimar (cork oak), which is a main forest species grown naturally in DPR Korea, with T. melanosporum mycelium to identify the emerged mycorrhizal association morphologically and molecularly. It was shown that Q. acutissimar, selected as the host species, was compatible with T. melanosporum. One hundred of the Q. acutissimar seeds were allowed to germinate and germination was observed in 84 seeds. Among 84 germinated seedlings, 70 seedlings with relatively good growth were selected and inoculated with T. melanosporum mycelium. Characteristic mycorrhizal structures between T. melanosporum and Quercus acutissimar were observed in 52 seedlings. The identification of T. melanosporum mycorrhiza was based on morphological and anatomical features and confirmed with molecular analyses by PCR. This is the first report on the establishment of the ECM association between T. melanosporum mycelia with Quercus acutissimar seedlings in DPR Korea.
},
year = {2025}
}
TY - JOUR T1 - Developments of Mycorrhiza in Quercus acutissimar (Cork Oak) Seedlings Inoculated with Black Truffle (Tuber melanosporum) Mycelial Pure Cultures AU - Kum-dong Jang AU - Yong-nam Kim AU - Chol Kim AU - Bok-sil Hyon AU - Hyok Kim AU - Jong-chong Kim AU - Hui-won Kim Y1 - 2025/11/07 PY - 2025 N1 - https://doi.org/10.11648/j.fem.20251103.12 DO - 10.11648/j.fem.20251103.12 T2 - Frontiers in Environmental Microbiology JF - Frontiers in Environmental Microbiology JO - Frontiers in Environmental Microbiology SP - 62 EP - 65 PB - Science Publishing Group SN - 2469-8067 UR - https://doi.org/10.11648/j.fem.20251103.12 AB - Tuber melanosporum (T. melanosporum), which is known as the black truffle, is the most famous edible ectomycorrhizal (ECM) fungus with a great commercial interest in the world, due to the expensive delicacies and can develop symbiotically with the roots of various trees and some bushes. T. melanosporum was ascomycete hypogeous fungi, which forms an underground fruitbody. In this study, we infected the seedlings of Quercus acutissimar (cork oak), which is a main forest species grown naturally in DPR Korea, with T. melanosporum mycelium to identify the emerged mycorrhizal association morphologically and molecularly. It was shown that Q. acutissimar, selected as the host species, was compatible with T. melanosporum. One hundred of the Q. acutissimar seeds were allowed to germinate and germination was observed in 84 seeds. Among 84 germinated seedlings, 70 seedlings with relatively good growth were selected and inoculated with T. melanosporum mycelium. Characteristic mycorrhizal structures between T. melanosporum and Quercus acutissimar were observed in 52 seedlings. The identification of T. melanosporum mycorrhiza was based on morphological and anatomical features and confirmed with molecular analyses by PCR. This is the first report on the establishment of the ECM association between T. melanosporum mycelia with Quercus acutissimar seedlings in DPR Korea. VL - 11 IS - 3 ER -
Department of Daily Food, Institute of Microbiology, Pyongyang, Democratic People’s Republic of Korea
