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Antibacterial Activity of Pteridophytes and Nigella sativa Against Antibiotic Resistant Bacteria Isolated from Wastewater Environment

Received: 29 August 2015     Accepted: 9 September 2015     Published: 11 September 2015
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Abstract

Bacterial resistance to antibiotics has become a global problem impacted partly by use of antibiotics in various environments. New antimicrobial agents are therefore urgently needed to overcome this problem. This study was carried out to investigate the tetracycline (TC) and ampicillin (AMP) resistant bacteria in wastewater environments of Rajshahi Metropolitan City, Bangladesh. In this investigation ethanol and acetone extracts of Dryopteris sp. (leaves), Pteris vittata L. (leaves) and Nigella sativa L. (seeds) were examined for their antibacterial activity. A total of 34 TC and AMP resistant bacteria were isolated from wastewater sources. Among them, a random selection of eight strains (three gram negative and 5 gram positive) was used for this study. The zone of inhibition for Dryopteris sp. leaves ranged from 6-11 mm and 6-12 mm and for Pteris vittata L. leaf extract, from 6-12 mm and 6-13 mm within ethanol and acetone extracts, respectively. Acetone extract of Nigella sativa L. seeds at the concentration of 2500 µg/ml had a marked sensitivity (7-18 mm) towards three tested gram positive bacteria in this study. The results of the present work provide essential baseline information for the use of the studied plants in the fight against drug resistant phenotypes.

Published in Frontiers in Environmental Microbiology (Volume 1, Issue 2)
DOI 10.11648/j.fem.20150102.14
Page(s) 27-31
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Antibacterial Activity, Pteridophytic Plants, Nigella sativa, Leaves Extracts, Antibiotic Resistant Bacteria

References
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Cite This Article
  • APA Style

    Farzana Ashrafi Neela, Rokshana Parvin, Niren Chandra Mahato, Md. Momtaz Uddin, Lipika Ghosh, et al. (2015). Antibacterial Activity of Pteridophytes and Nigella sativa Against Antibiotic Resistant Bacteria Isolated from Wastewater Environment. Frontiers in Environmental Microbiology, 1(2), 27-31. https://doi.org/10.11648/j.fem.20150102.14

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    ACS Style

    Farzana Ashrafi Neela; Rokshana Parvin; Niren Chandra Mahato; Md. Momtaz Uddin; Lipika Ghosh, et al. Antibacterial Activity of Pteridophytes and Nigella sativa Against Antibiotic Resistant Bacteria Isolated from Wastewater Environment. Front. Environ. Microbiol. 2015, 1(2), 27-31. doi: 10.11648/j.fem.20150102.14

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    AMA Style

    Farzana Ashrafi Neela, Rokshana Parvin, Niren Chandra Mahato, Md. Momtaz Uddin, Lipika Ghosh, et al. Antibacterial Activity of Pteridophytes and Nigella sativa Against Antibiotic Resistant Bacteria Isolated from Wastewater Environment. Front Environ Microbiol. 2015;1(2):27-31. doi: 10.11648/j.fem.20150102.14

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  • @article{10.11648/j.fem.20150102.14,
      author = {Farzana Ashrafi Neela and Rokshana Parvin and Niren Chandra Mahato and Md. Momtaz Uddin and Lipika Ghosh and Most. Ferdousi Begum},
      title = {Antibacterial Activity of Pteridophytes and Nigella sativa Against Antibiotic Resistant Bacteria Isolated from Wastewater Environment},
      journal = {Frontiers in Environmental Microbiology},
      volume = {1},
      number = {2},
      pages = {27-31},
      doi = {10.11648/j.fem.20150102.14},
      url = {https://doi.org/10.11648/j.fem.20150102.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20150102.14},
      abstract = {Bacterial resistance to antibiotics has become a global problem impacted partly by use of antibiotics in various environments. New antimicrobial agents are therefore urgently needed to overcome this problem. This study was carried out to investigate the tetracycline (TC) and ampicillin (AMP) resistant bacteria in wastewater environments of Rajshahi Metropolitan City, Bangladesh. In this investigation ethanol and acetone extracts of Dryopteris sp. (leaves), Pteris vittata L. (leaves) and Nigella sativa L. (seeds) were examined for their antibacterial activity. A total of 34 TC and AMP resistant bacteria were isolated from wastewater sources. Among them, a random selection of eight strains (three gram negative and 5 gram positive) was used for this study. The zone of inhibition for Dryopteris sp. leaves ranged from 6-11 mm and 6-12 mm and for Pteris vittata L. leaf extract, from 6-12 mm and 6-13 mm within ethanol and acetone extracts, respectively. Acetone extract of Nigella sativa L. seeds at the concentration of 2500 µg/ml had a marked sensitivity (7-18 mm) towards three tested gram positive bacteria in this study. The results of the present work provide essential baseline information for the use of the studied plants in the fight against drug resistant phenotypes.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - Antibacterial Activity of Pteridophytes and Nigella sativa Against Antibiotic Resistant Bacteria Isolated from Wastewater Environment
    AU  - Farzana Ashrafi Neela
    AU  - Rokshana Parvin
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    AU  - Md. Momtaz Uddin
    AU  - Lipika Ghosh
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    DO  - 10.11648/j.fem.20150102.14
    T2  - Frontiers in Environmental Microbiology
    JF  - Frontiers in Environmental Microbiology
    JO  - Frontiers in Environmental Microbiology
    SP  - 27
    EP  - 31
    PB  - Science Publishing Group
    SN  - 2469-8067
    UR  - https://doi.org/10.11648/j.fem.20150102.14
    AB  - Bacterial resistance to antibiotics has become a global problem impacted partly by use of antibiotics in various environments. New antimicrobial agents are therefore urgently needed to overcome this problem. This study was carried out to investigate the tetracycline (TC) and ampicillin (AMP) resistant bacteria in wastewater environments of Rajshahi Metropolitan City, Bangladesh. In this investigation ethanol and acetone extracts of Dryopteris sp. (leaves), Pteris vittata L. (leaves) and Nigella sativa L. (seeds) were examined for their antibacterial activity. A total of 34 TC and AMP resistant bacteria were isolated from wastewater sources. Among them, a random selection of eight strains (three gram negative and 5 gram positive) was used for this study. The zone of inhibition for Dryopteris sp. leaves ranged from 6-11 mm and 6-12 mm and for Pteris vittata L. leaf extract, from 6-12 mm and 6-13 mm within ethanol and acetone extracts, respectively. Acetone extract of Nigella sativa L. seeds at the concentration of 2500 µg/ml had a marked sensitivity (7-18 mm) towards three tested gram positive bacteria in this study. The results of the present work provide essential baseline information for the use of the studied plants in the fight against drug resistant phenotypes.
    VL  - 1
    IS  - 2
    ER  - 

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Author Information
  • Department of Botany, Rajshahi University, Rajshahi, Bangladesh

  • Department of Botany, Rajshahi University, Rajshahi, Bangladesh

  • Department of Botany, Rajshahi University, Rajshahi, Bangladesh

  • Department of Botany, Rajshahi University, Rajshahi, Bangladesh

  • Department of Botany, Rajshahi University, Rajshahi, Bangladesh

  • Department of Botany, Rajshahi University, Rajshahi, Bangladesh

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