Volume 6, Issue 3, September 2020, Page: 40-51
Effect of Trichoderma asperellum and Trichoderma virens on Allium cepa L. Growth, Damping off and Basal Rot Disease Incidence and Severity in Sri Lanka
Lankeswarage Nilupa Rasangi Gunaratna, Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo, Sri Lanka
Nelum Deshappriya, Department of Botany, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka
Sirimewan Rajapaksha, Horticultural Crops Research and Development Institute, Gannoruwa, Peradeniya, Sri Lanka
Dehiwala Liyanage Jayaratne, Department of Microbiology, Faculty of Science, University of Kelaniya, Kelaniya, Sri Lanka
Received: Jun. 24, 2020;       Accepted: Sep. 2, 2020;       Published: Sep. 23, 2020
DOI: 10.11648/j.fem.20200603.13      View  47      Downloads  26
Abstract
Trichoderma species are frequently used for the biological control of phytopathogenic fungi and they have also been reported as plant growth promoters. In the present study, the effect of two Trichoderma spp. i.e. Trichoderma asperellum and Trichoderma virens isolated from the soils of onion fields on the growth of Allium cepa L. plants and suppression of damping off and basal rot diseases was evaluated under field conditions. The two Trichoderma spp. were mass cultured in a low cost medium containing molasses and yeast and added to a low cost carrier medium consisting of talc. Two formulations, i.e. T. asperellum only and T. asperellum in combination with T. virens were prepared and the formulations were tested for their effect on onion seedlings at the nursery stage and also on transplanted plants in the field. At the nursery stage, the two formulations were applied using two methods i.e. soil application prior to planting of onion seeds or priming of onion seeds with the two formulations separately before planting. Both methods reduced the incidence and severity of damping off disease while increasing the growth of seedlings significantly (p ≤ 0.05) at the nursery stage. Additional treatment with the two formulations as seedling root dips or soil applications before transplanting the seedlings in the field were effective in controlling basal rot disease of A. cepa L. and enhancing the growth of Allium cepa L. plants significantly (p ≤ 0.05) in the field.
Keywords
Trichoderma asperellum, Trichoderma virens, Damping Off, Basal Rot, Growth Enhancement
To cite this article
Lankeswarage Nilupa Rasangi Gunaratna, Nelum Deshappriya, Sirimewan Rajapaksha, Dehiwala Liyanage Jayaratne, Effect of Trichoderma asperellum and Trichoderma virens on Allium cepa L. Growth, Damping off and Basal Rot Disease Incidence and Severity in Sri Lanka, Frontiers in Environmental Microbiology. Vol. 6, No. 3, 2020, pp. 40-51. doi: 10.11648/j.fem.20200603.13
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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