Volume 6, Issue 1, March 2020, Page: 5-10
Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki
Adeoti Olatunde Micheal, Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic Saki, Saki, Oyo State, Nigeria
Akinbo Kehinde Oluwabusayo, Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic Saki, Saki, Oyo State, Nigeria
Adesina David Ademola, Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic Saki, Saki, Oyo State, Nigeria
Olufemi Samson Olutope, Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic Saki, Saki, Oyo State, Nigeria
Received: Dec. 23, 2019;       Accepted: Jan. 16, 2020;       Published: Apr. 8, 2020
DOI: 10.11648/j.fem.20200601.12      View  358      Downloads  72
Abstract
Water security and safety is of vital concern in Saki and the rest of the world. The aim of this was to screen major sources of water in Saki of potential pathogens of public health importance. Water samples of six different water sources were purposely collected aseptically from six different locations in Saki (well water, river water, rain water, borehole water, sachet water and bottled water); these were subjectsed to standard laboratory analysis. Four highly populated plates were sent for molecular characterization for Polymerase Chain Reaction and species sequencing for molecular identification. The highest total colony count was 70.0×10-5 from river water followed by rain water with the lowest of 0.1×105 CFU from borehole water. Molecular analysis identified amplification of isolates genes at 15000 base pair for river water and well water samples. The analysis showed that Klebsiella pneumonia (MN208186) and Enterobacter kobeii (KM593928) were the organisms found in rain water and river water respectively. The resulting nucleotide sequences was blasted at NCBI for pairwise comparison with existing data in the GenBank and their similarity was noted. The pathogenicity of the two isolates have been well-documented, making some of the water sources tested so unfit for human usage. Large-scale treatment of water sources are recommended before consumption viz-a-vis hygienic practices around water bodies.
Keywords
Fecal Coliform, Aseptically, Blasted, Enterobacter Kobeii, Water-borne Diseases, Well Water
To cite this article
Adeoti Olatunde Micheal, Akinbo Kehinde Oluwabusayo, Adesina David Ademola, Olufemi Samson Olutope, Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki, Frontiers in Environmental Microbiology. Vol. 6, No. 1, 2020, pp. 5-10. doi: 10.11648/j.fem.20200601.12
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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